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KMID : 0383820100690050348
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Tuberculosis and Respiratory Diseases
2010 Volume.69 No. 5 p.348 ~ p.353
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Regulation of Tumor Necrosis Factor-α-induced Airway Mucin Production and Gene Expression by Carbenoxolone, Prunetin, and Silibinin
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Lee Hyun-Jae
Lee Su-Yel
Jeon Byeong-Kyou
Lee Jae-Woo
Lee Mi-Nam
Kim Ju-Ock
Lee Choong-Jae
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Abstract
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Background: In this study, we tried to investigate whether carbenoxolone, prunetin, and silibinin affect tumor necrosis factor (TNF)-α- induced MUC5AC mucin production and gene expression from human airway epithelial cells.
Materials and Methods : Confluent NCI-H292 cells were pretreated with each agent (carbenoxolone, prunetin, and silibinin) for 30 min and then stimulated with TNF-α for 24 hours. The MUC5AC mucin gene expression and mucin protein production were measured by reverse transcription-polymerase chain reaction and enzyme linked immunosorbent assay, respectively.
Results: Carbenoxolone, prunetin and silibinin inhibited the production of MUC5AC mucin protein induced by TNF-α; the 3 compounds also inhibited the expression of MUC5AC mucin gene induced by TNF-α.
Conclusion: This result suggests that carbenoxolone, prunetin and silibinin can inhibit mucin gene expression and production of mucin protein induced by TNF-α, by directly acting on airway epithelial cells.
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KEYWORD
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MUC5AC protein, human, Carbenoxolone, Prunetin, Silibinin
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